Are substrate use during exercise and mitochondrial respiratory capacity decreased in arm and leg muscle in type 2 diabetes?
- 25 April 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Diabetologia
- Vol. 52 (7), 1400-1408
- https://doi.org/10.1007/s00125-009-1353-4
Abstract
Aim/hypothesis The aim of the study was to investigate mitochondrial function, fibre type distribution and substrate oxidation in arm and leg muscle during exercise in patients with type 2 diabetes and in obese and lean controls. Methods Indirect calorimetry was used to calculate fat and carbohydrate oxidation during both progressive arm-cranking and leg-cycling exercises. Muscle biopsies from arm and leg were obtained. Fibre type, as well as O2 flux capacity of saponin-permeabilised muscle fibres were measured, the latter by high resolution respirometry, in patients with type 2 diabetes, age- and BMI-matched obese controls, and age-matched lean controls. Results Fat oxidation was similar in the groups during either arm or leg exercise. During leg exercise at higher intensities, but not during arm exercise, carbohydrate oxidation was lower in patients with type 2 diabetes compared with the other groups. In patients with type 2 diabetes, ADP-stimulated state 3 respiration per mg muscle with parallel electron input from complex I+II was lower in m. vastus lateralis compared with obese and lean controls, whereas no differences between groups were present in m. deltoideus. A higher percentage of type IIX fibres was seen in m. vastus lateralis in patients with type 2 diabetes compared with obese and lean controls, whereas no difference was found in the deltoid muscle. Conclusions/interpretation This study demonstrates similar O2 flux capacity, fibre type distribution and carbohydrate oxidation in arm muscle in the groups despite the presence of attenuated values in leg muscle in patients with type 2 diabetes compared with obese and lean controls.Keywords
This publication has 44 references indexed in Scilit:
- Lower Intrinsic ADP-Stimulated Mitochondrial Respiration Underlies In Vivo Mitochondrial Dysfunction in Muscle of Male Type 2 Diabetic PatientsDiabetes, 2008
- Morphology of the deltoid muscles in elite tennis playersJournal of Sports Sciences, 2007
- Patients with type 2 diabetes have normal mitochondrial function in skeletal muscleDiabetologia, 2007
- Substrate oxidation during exercise: type 2 diabetes is associated with a decrease in lipid oxidation and an earlier shift towards carbohydrate utilizationDiabetes & Metabolism, 2006
- The Effect of Crank Rate on Physiological Responses and Exercise Efficiency Using a Range of Submaximal Workloads During Arm Crank ErgometryInternational Journal of Sports Medicine, 2005
- Resistance training and insulin action in humans: effects of de-trainingThe Journal of Physiology, 2003
- Effects of bedrest on deltoideus muscle morphology and enzymesActa Physiologica Scandinavica, 1998
- Impaired free fatty acid utilization by skeletal muscle in non-insulin-dependent diabetes mellitus.JCI Insight, 1994
- Design of the mammalian respiratory system. IX. Functional and structural limits for oxygen flowRespiration Physiology, 1981
- Muscle Metabolites during Submaximal and Maximal Exercise in ManScandinavian Journal of Clinical and Laboratory Investigation, 1970