Acute myocardial infarction in mice: assessment of transmurality by strain rate imaging
Open Access
- 1 July 2007
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 293 (1), H496-H502
- https://doi.org/10.1152/ajpheart.00087.2007
Abstract
In vivo evaluation of the transmural extension of myocardial infarction (TEI) is crucial to prediction of viability and prognosis. With the rise of transgenic technology, murine myocardial infarction (MI) models are increasingly used. Our study aimed to evaluate systolic strain rate (SR), a new parameter of regional function, to quantify TEI in a murine model of acute MI induced by various durations of ischemia followed by 24 h of reperfusion. Global and regional left ventricular (LV) function were assessed by echocardiography (13 MHz, Vivid 7, GE) in 4 groups of wild-type mice (C57BL/6, 2 mo old): a sham-treated group ( n = 10) and three MI groups [30 ( n = 11), 60 ( n = 10), and 90 ( n = 9) min of left coronary artery occlusion]. Conventional LV dimensions, anterior wall (AW) thickening, and peak systolic SR were measured before and 24 h after reperfusion. Area at risk (AR) was measured by blue dye and infarct size (area of necrosis, AN) and TEI by triphenyltetrazolium chloride staining. AN increased with ischemia duration (25 ± 2%, 56 ± 5%, 71 ± 6% of AR for 30, 60, and 90 min, respectively; P < 0.05). LV end-diastolic volume significantly increased with ischemia duration (30 ± 5, 34 ± 5, 43 ± 5 μl; P < 0.05), whereas LV ejection fraction decreased (63 ± 5%, 58 ± 6%, 46 ± 5%; P < 0.05). AW thickening decrease was not influenced by ischemia duration. Conversely, systolic SR decreased with ischemia duration (13 ± 5, 4 ± 3, −2 ± 6 s−1; P < 0.05) and was significantly correlated with TEI ( r = 0.89, P < 0.01). Receiver operating characteristic (ROC) curves identified systolic SR as the most accurate parameter to predict TEI. In conclusion, in a murine model of MI, SR imaging is superior to conventional echocardiography to predict TEI early after MI.Keywords
This publication has 45 references indexed in Scilit:
- Systematic evaluation of a novel model for cardiac ischemic preconditioning in miceAmerican Journal of Physiology-Heart and Circulatory Physiology, 2006
- Coronary flow reserve and heart failure in experimental coxsackievirus myocarditis. A transthoracic Doppler echocardiography studyAmerican Journal of Physiology-Heart and Circulatory Physiology, 2006
- Sustained augmentation of cardiac α1A-adrenergic drive results in pathological remodeling with contractile dysfunction, progressive fibrosis and reactivation of matricellular protein genesJournal of Molecular and Cellular Cardiology, 2006
- A novel model of cryoinjury-induced myocardial infarction in the mouse: a comparison with coronary artery ligationAmerican Journal of Physiology-Heart and Circulatory Physiology, 2005
- Simultaneous assessment of left‐ventricular infarction size, function and tissue viability in a murine model of myocardial infarction by cardiac manganese‐enhanced magnetic resonance imaging (MEMRI)NMR in Biomedicine, 2004
- Impact of scar thickness on the assessment of viability using dobutamine echocardiography and thallium single-photon emission computed tomography: A comparison with contrast-enhanced magnetic resonance imagingJournal of the American College of Cardiology, 2004
- Strain rate measurement by doppler echocardiography allows improvedassessment of myocardial viability inpatients with depressed left ventricular functionJournal of the American College of Cardiology, 2002
- IntroductionThe American Journal of Cardiology, 1991
- IntroductionThe American Journal of Cardiology, 1991
- Two-dimensional echocardiography and infarct size: relationship of regional wall motion and thickening to the extent of myocardial infarction in the dog.Circulation, 1981