Endotoxin and cytokines induce expression of leptin, the ob gene product, in hamsters.
Open Access
- 1 May 1996
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 97 (9), 2152-2157
- https://doi.org/10.1172/jci118653
Abstract
The expression of leptin, the ob gene product, is increased in adipose tissue in response to feeding and energy repletion, while leptin decreases food intake. Because adipose tissue gene expression is regulated by cytokines induced during infection and because infection is associated with anorexia, we tested whether induction of leptin might occur during the host response to infection. Administration of endotoxin (LPS), a model for gram negative infections, induces profound anorexia and weight loss in hamsters. In fasted adipose tissue to levels similar to fed control animals. There is a strong inverse correlation between mRNA levels of leptin and subsequent food intake. TNF and IL-1, mediators of the host response to LPS, also induced anorexia and increased levels of leptin in mRNA in adipose tissue. As assessed by immuknoprecipitation and Western blotting, circulating leptin protein is regulated by LPS and cytokines in parallel to regulation of adipose tissue leptin mRNA. Induction of leptin during the host response to infection may contribute to the anorexia of infection.Keywords
This publication has 45 references indexed in Scilit:
- The role of neuropeptide Y in the antiobesity action of the obese gene productNature, 1995
- Transient increase in obese gene expression after food intake or insulin administrationNature, 1995
- Weight-Reducing Effects of the Plasma Protein Encoded by the obese GeneScience, 1995
- Effects of the obese Gene Product on Body Weight Regulation in ob / ob MiceScience, 1995
- The role of cholecystokinin in interleukin-1-induced anorexiaPhysiology & Behavior, 1993
- Cachexia and the acute‐phase protein response in inflammation are regulated by interleukin‐6European Journal of Immunology, 1993
- Both cyclooxygenase and lipoxygenase inhibitor partially restore the anorexia by interleukin-1βLife Sciences, 1992
- Tackling a weighty problemCell, 1992
- Cachectin/tumor necrosis factor induces cachexia, anemia, and inflammation.The Journal of Experimental Medicine, 1988
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987