From small hosts come big viruses: the complete genome of a second Ostreococcus tauri virus, OtV‐1
Open Access
- 27 October 2009
- journal article
- Published by Wiley in Environmental Microbiology
- Vol. 11 (11), 2821-2839
- https://doi.org/10.1111/j.1462-2920.2009.01991.x
Abstract
Ostreococcus tauri virus (OtV-1) is a large double-stranded DNA virus and a prospective member of the family Phycodnaviridae, genus Prasinovirus. OtV-1 infects the unicellular marine green alga O. tauri, the smallest known free-living eukaryote. Here we present the 191 761 base pair genome sequence of OtV-1, which has 232 putative protein-encoding and 4 tRNA-encoding genes. Approximately 31% of the viral gene products exhibit a similarity to proteins of known functions in public databases. These include a variety of unexpected genes, for example, a PhoH-like protein, a N-myristoyltransferase, a 3-dehydroquinate synthase, a number of glycosyltransferases and methyltransferases, a prolyl 4-hydroxylase, 6-phosphofructokinase and a total of 8 capsid proteins. A total of 11 predicted genes share homology with genes found in the Ostreococcus host genome. In addition, an intein was identified in the DNA polymerase gene of OtV-1. This is the first report of an intein in the genome of a virus that infects O. tauri. Fifteen core genes common to nuclear-cytoplasmic large dsDNA virus (NCLDV) genomes were identified in the OtV-1 genome. This new sequence data may help to redefine the classification of the core genes of these viruses and shed new light on their evolutionary history.Keywords
This publication has 103 references indexed in Scilit:
- Role of unusual O-glycans in intercellular signalingThe International Journal of Biochemistry & Cell Biology, 2009
- An original adaptation of photosynthesis in the marine green alga OstreococcusProceedings of the National Academy of Sciences of the United States of America, 2008
- Life-Cycle and Genome of OtV5, a Large DNA Virus of the Pelagic Marine Unicellular Green Alga Ostreococcus tauriPLOS ONE, 2008
- Structure and Function of a Chlorella Virus-Encoded GlycosyltransferaseStructure, 2007
- Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfaceaVirology, 2007
- Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64AVirology, 2007
- Identifying bacterial genes and endosymbiont DNA with GlimmerBioinformatics, 2007
- Three Prochlorococcus Cyanophage Genomes: Signature Features and Ecological InterpretationsPLoS Biology, 2005
- tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic SequenceNucleic Acids Research, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994