Construction and Recovery of Viable Retroviral Genomes Carrying a Bacterial Suppressor Transfer RNA Gene
- 19 April 1985
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 228 (4697), 329-332
- https://doi.org/10.1126/science.2984770
Abstract
The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.Keywords
This publication has 34 references indexed in Scilit:
- Activation of the cellular oncogene c-erbB by ltr insertion: Molecular basis for induction of erythroblastosis by avian leukosis virusCell, 1983
- Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cellsNature, 1982
- Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virusNature, 1982
- Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genomeNature, 1981
- Retroviruses as mutagens: Insertion and excision of a nontransforming provirus alter expression of a resident transforming provirusCell, 1981
- Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosisNature, 1981
- The integrated genome of murine leukemia virusCell, 1978
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolutionJournal of Molecular Biology, 1975
- Selective extraction of polyoma DNA from infected mouse cell culturesJournal of Molecular Biology, 1967