Environmental oxygen tension affects phenotype in cultured bone marrow-derived macrophages
Open Access
- 1 February 2004
- journal article
- Published by American Physiological Society in American Journal of Physiology-Lung Cellular and Molecular Physiology
- Vol. 286 (2), L354-L362
- https://doi.org/10.1152/ajplung.00380.2002
Abstract
This study tested the hypothesis that the unique phenotype of alveolar macrophages (AM) is maintained through adaptation to the relatively high oxygen partial pressure (Po2) of the lung, through modification of redox-sensitive transcription factors. BALB/c mouse bone marrowderived macrophages (BMC) were differentiated under different Po2and compared functionally to AM and peritoneal macrophages (PM). BMC differentiated in normoxia (Po2140 Torr, BMChigh) were similar to AM in having low phagocytic and antigen presenting cell (APC) activities. However, BMC grown in low oxygen tension as found in other tissues (low) were better phagocytes and APCs, similar to PM. BMChighwere more oxidative intracellularly than BMClow, based on oxidation of dichlorofluorescein and higher glutathione disulfide/glutathione (GSH) ratios, despite having more GSH. Finally, lipopolysaccharide-induced nuclear factor-κB translocation, measured by laser scanning cytometry, was reduced in BMChighand AM, compared with BMClowand PM, respectively. These data suggest that regulation of the AM phenotype may occur, at least in part, via inhibition of NF-κB by the unique redox environment.Keywords
This publication has 20 references indexed in Scilit:
- The polarization of Th1/Th2 balance is dependent on the intracellular thiol redox status of macrophages due to the distinctive cytokine productionInternational Immunology, 2002
- Cytokine-induced Activation of Nuclear Factor-κB Is Inhibited by Hydrogen Peroxide through Oxidative Inactivation of IκB KinaseJournal of Biological Chemistry, 2001
- Effective antigen presentation by dendritic cells is NF-κB dependent: coordinate regulation of MHC, co-stimulatory molecules and cytokinesInternational Immunology, 2001
- The Activity of NF-kappaB in Swiss 3T3 Cells Exposed to Aqueous Extracts of Cigarette Smoke Is Dependent on ThioredoxinToxicological Sciences, 2001
- Regulation of nuclear factor-κB, activator protein-1, and glutathione levels by tumor necrosis factor-α and dexamethasone in alveolar epithelial cellsBiochemical Pharmacology, 2000
- Oxidative stress and nuclear factor-κB activationBiochemical Pharmacology, 2000
- Human alveolar macrophages present antigen ineffectively due to defective expression of B7 costimulatory cell surface molecules.JCI Insight, 1995
- Regulation of the transcription factors NF-κB and AP-1 by redox changesChemico-Biological Interactions, 1994
- Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages.The Journal of Experimental Medicine, 1993
- Enzymatic basis for bioenergetic differences of alveolar versus peritoneal macrophages and enzyme regulation by molecular O2.JCI Insight, 1977