Exposure to lanthanum compound diminishes LPS-induced inflammation-associated gene expression: involvements of PKC and NF-κB signaling pathways
- 27 March 2010
- journal article
- Published by Springer Science and Business Media LLC in BioMetals
- Vol. 23 (4), 669-680
- https://doi.org/10.1007/s10534-010-9327-z
Abstract
Lanthanum chloride, a rare earth compound, possesses antibacterial and cellular immunity regulating properties. However, the underlying molecular mechanisms remain largely unknown. In this study, we examined the effects of lanthanum chloride on the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α), the expression of inducible NO synthase (iNOS) and TNF-α in RAW 264.7 cells, a mouse macrophage cell line. We found that the LPS-elicited excessive production of NO and TNF-α in RAW 264.7 cells was inhibited significantly in the presence of lanthanum chloride, and the attenuation of iNOS and TNF-α occurred at mRNA level. Furthermore, the possible signaling components affected by lanthanum chloride in the pathway that lead to LPS-induced iNOS and TNF-α expression were explored. The results indicated the involvements of PKC/Ca2+ and NF-κB in the attenuation of NO and pro-inflammatory cytokine production by lanthanum chloride. Our observations suggest a possible therapeutic application of this agent for treating inflammatory diseases.Keywords
This publication has 47 references indexed in Scilit:
- Can the Risk of Gadolinium be Extrapolated to Lanthanum?Seminars in Dialysis, 2008
- NF-κB activation by depolarization of skeletal muscle cells depends on ryanodine and IP3 receptor-mediated calcium signalsAmerican Journal of Physiology-Cell Physiology, 2007
- Lanthanum Induces Extracellular Signal-regulated Kinase Phosphorylation through Different Mechanisms in HeLa Cells and NIH 3T3 CellsBioMetals, 2006
- Antihuman Immunodeficiency Virus Type 1 (HIV-1) Activity of Rare Earth Metal Complexes of 4-Hydroxycoumarins in Cell CultureBioinorganic Chemistry and Applications, 2006
- Gold compound auranofin inhibits IκB kinase (IKK) by modifying Cys-179 of IKKβ subunitExperimental & Molecular Medicine, 2003
- Sustained Signaling by Phospholipase C-γ Mediates Nerve Growth Factor-Triggered Gene ExpressionMolecular and Cellular Biology, 2001
- Effects of cerium nitrate bathing and prompt burn wound excision on IL-6 and TNF-α levels in burned ratsBurns, 2000
- Involvement of protein kinase C in superoxide anion-induced activation of nuclear factor-κB in human endothelial cellsCardiovascular Research, 2000
- The Transcriptional Activity of NF-κB Is Regulated by the IκB-Associated PKAc Subunit through a Cyclic AMP–Independent MechanismCell, 1997
- Essential Role of NF-κB Activation in Silica-Induced Inflammatory Mediator Production in MacrophagesBiochemical and Biophysical Research Communications, 1995