Nectin 4 Overexpression in Ovarian Cancer Tissues and Serum
Open Access
- 1 November 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 134 (5), 835-845
- https://doi.org/10.1309/ajcpgxk0fr4mhihb
Abstract
Early detection of ovarian cancer is difficult owing to the lack of specific and sensitive tests available. Previously, we found expression of nectin 4 to be increased in ovarian cancer compared with normal ovaries. Reverse transcriptase–polymerase chain reaction (RT-PCR) and quantitative RT-PCR validated the overexpression of nectin 4 messenger RNA in ovarian cancer compared with normal ovarian cell lines and tissues. Protein levels of nectin 4 were elevated in ovarian cancer cell lines and tissue compared with normal ovarian cell lines as demonstrated by Western immunoblotting, flow cytometry, and immunohistochemical staining of tissue microarray slides. Cleaved nectin 4 was detectable in a number of patient serum samples by enzyme-linked immunosorbent assay. In patients with benign gynecologic diseases with high serum CA125 levels, nectin 4 was not detected in the majority of cases, suggesting that nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125.Keywords
This publication has 33 references indexed in Scilit:
- Identification of candidate biomarkers in ovarian cancer serum by depletion of highly abundant proteins and differential in‐gel electrophoresisElectrophoresis, 2010
- S100A1 Expression in Ovarian and Endometrial Endometrioid Carcinomas Is a Prognostic Indicator of Relapse-Free SurvivalAmerican Journal of Clinical Pathology, 2009
- Identification of Nectin-4 Oncoprotein as a Diagnostic and Therapeutic Target for Lung CancerCancer Research, 2009
- Histologic Subtypes of Ovarian CarcinomaInternational Journal of Gynecological Pathology, 2008
- Cancer Statistics, 2008CA: A Cancer Journal for Clinicians, 2008
- My approach to and thoughts on the typing of ovarian carcinomasJournal of Clinical Pathology, 2007
- Nectin-4 is a new histological and serological tumor associated marker for breast cancerBMC Cancer, 2007
- Roles of nectins in cell adhesion, migration and polarizationBiological Chemistry, 2004
- Role of each immunoglobulin-like loop of nectin for its cell–cell adhesion activityBiochemical and Biophysical Research Communications, 2003
- Nectin4/PRR4, a New Afadin-associated Member of the Nectin Family That Trans-interacts with Nectin1/PRR1 through V Domain InteractionOnline Journal of Public Health Informatics, 2001