Intracellular Localization of the Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein: Absence of Nucleolar Accumulation during Infection and after Expression as a Recombinant Protein in Vero Cells
- 1 September 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (17), 11507-12
- https://doi.org/10.1128/jvi.79.17.11507-11512.2005
Abstract
The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.Keywords
This publication has 23 references indexed in Scilit:
- Colocalization and Interaction of the Porcine Arterivirus Nucleocapsid Protein with the Small Nucleolar RNA-Associated Protein FibrillarinJournal of Virology, 2003
- The nucleolus – a gateway to viral infection?Archiv für die gesamte Virusforschung, 2002
- Interaction of the Coronavirus Nucleoprotein with Nucleolar Antigens and the Host CellJournal of Virology, 2002
- Localization to the Nucleolus Is a Common Feature of Coronavirus Nucleoproteins, and the Protein May Disrupt Host Cell DivisionJournal of Virology, 2001
- Biophysical Characterization of Interactions Involving Importin-α during Nuclear ImportPublished by Elsevier BV ,2001
- Sequence and structure-based prediction of eukaryotic protein phosphorylation sitesJournal of Molecular Biology, 1999
- Transport Between the Cell Nucleus and the CytoplasmAnnual Review of Cell and Developmental Biology, 1999
- Nuclear and nucleolar targeting of human ribosomal protein S25: Common features shared with HIV-1 regulatory proteinsOncogene, 1999
- Two nucleolar targeting signals present in the N-terminal part of Semliki Forest virus capsid proteinArchiv für die gesamte Virusforschung, 1994
- Functional similarity of HIV-I rev and HTLV-I rex proteins: Identification of a new nucleolar-targeting signal in rev proteinBiochemical and Biophysical Research Communications, 1989