Selective targeting of miRNA‐regulated plant development by a viral counter‐silencing protein
- 21 March 2007
- journal article
- Published by Wiley in The Plant Journal
- Vol. 50 (2), 240-252
- https://doi.org/10.1111/j.1365-313x.2007.03042.x
Abstract
13 páginas, 5 figuras, 2 tablas -- PAGS nros. 240-252The cucumber mosaic virus (CMV) 2b protein suppresses RNA silencing and determines viral symptoms. Among Arabidopsis thaliana lines expressing 2b proteins from mild (LS and Q CMV) or severe (Fny CMV) strains, only Fny 2b-transgenic plants displayed strong symptom-like phenotypes in leaves, stems and flowers, together with stunting of main root growth and increased emergence of lateral roots. However, LS and Fny 2b proteins both enhanced lateral root length. Micro (mi)RNA-mediated cellular mRNA turnover was inhibited in Fny 2b-transgenic plants, but there was no evidence for this in LS 2b-transgenic plants. Both 2b proteins efficiently suppressed small interfering (si)RNA-mediated RNA silencing, suggesting that 2b proteins can target the siRNA pathway without disrupting miRNA-regulated RNA turnover. Thus, symptom induction is not an inevitable consequence of RNA silencing suppression. For CMV, strain-specific differences between the 2b silencing proteins determine whether only one or both small RNA-guided RNA destruction pathways are disruptedM.L. was supported by a Biotechnological and Biological Sciences Research Council (BBSRC) studentship, and F.C.R. by a studentship from the Gatsby Charitable Trust. J.P.C.’s laboratory is funded by BBSRC grant BB/D008204/1, and work by P.P. and T.C. is funded by the Scottish Executive Environment and Rural Affairs DepartmentPeer revieweKeywords
This publication has 63 references indexed in Scilit:
- Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defenseGenes & Development, 2006
- Hierarchical Action and Inhibition of Plant Dicer-Like Proteins in Antiviral DefenseScience, 2006
- Double-Stranded RNA Binding May Be a General Plant RNA Viral Strategy To Suppress RNA SilencingJournal of Virology, 2006
- Spatio‐temporal accumulation of microRNAs is highly coordinated in developing plant tissuesThe Plant Journal, 2006
- Induction and suppression of RNA silencing: insights from viral infectionsNature Reviews Genetics, 2005
- CARPEL FACTORY, a Dicer Homolog, and HEN1, a Novel Protein, Act in microRNA Metabolism in Arabidopsis thalianaCurrent Biology, 2002
- MicroRNAs in plantsGenes & Development, 2002
- Inhibition of tobacco mosaic virus replication in lateral roots is dependent on an activated meristem-derived signalProtoplasma, 2002
- Gene Silencing without DNA: RNA-Mediated Cross-Protection between VirusesTHE PLANT CELL ONLINE, 1999
- Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector.THE PLANT CELL ONLINE, 1995