Human Milk Oligosaccharides Promote the Growth of Staphylococci
Open Access
- 15 July 2012
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (14), 4763-4770
- https://doi.org/10.1128/aem.00477-12
Abstract
Human milk oligosaccharides (HMO), which constitute a major component of human milk, promote the growth of particular bacterial species in the infant's gastrointestinal tract. We hypothesized that HMO also interact with the bacterial communities present in human milk. To test this hypothesis, two experiments were conducted. First, milk samples were collected from healthy women ( n = 16); culture-independent analysis of the bacterial communities was performed, HMO content was analyzed, and the relation between these factors was investigated. A positive correlation was observed between the relative abundance of Staphylococcus and total HMO content ( r = 0.66). In a follow-up study, we conducted a series of in vitro growth curve experiments utilizing Staphylococcus aureus or Staphylococcus epidermidis and HMO isolated from human milk. HMO exhibited stimulatory effects on bacterial growth under various nutritional conditions. Analysis of culture supernatants from these experiments revealed that HMO did not measurably disappear from the culture medium, indicating that the growth-enhancing effects were not a result of bacterial metabolism of the HMO. Instead, stimulation of growth caused greater utilization of amino acids in minimal medium. Collectively, the data provide evidence that HMO may promote the growth of Staphylococcus species in the lactating mammary gland.Keywords
This publication has 41 references indexed in Scilit:
- Comparative Metabolic Flux Profiling of Melanoma Cell LinesJournal of Biological Chemistry, 2011
- Bacteroides in the Infant Gut Consume Milk Oligosaccharides via Mucus-Utilization PathwaysCell Host & Microbe, 2011
- Physiology of Consumption of Human Milk Oligosaccharides by Infant Gut-associated BifidobacteriaJournal of Biological Chemistry, 2011
- Annotation and Structural Analysis of Sialylated Human Milk OligosaccharidesJournal of Proteome Research, 2010
- Development of an Annotated Library of Neutral Human Milk OligosaccharidesJournal of Proteome Research, 2010
- Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharidesTrends in Microbiology, 2010
- Consumption of Human Milk Oligosaccharides by Gut-Related MicrobesJournal of Agricultural and Food Chemistry, 2010
- The Carbohydrate-Active EnZymes database (CAZy): an expert resource for GlycogenomicsNucleic Acids Research, 2008
- Staphylococcus epidermidis: A differential trait of the fecal microbiota of breast-fed infantsBMC Microbiology, 2008
- Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial TaxonomyApplied and Environmental Microbiology, 2007