Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
Top Cited Papers
- 1 March 2013
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 339 (6123), 1074-1077
- https://doi.org/10.1126/science.1232542
Abstract
Genomic enhancers are important regulators of gene expression, but their identification is a challenge, and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, which enables screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type–specific enhancers across a broad continuum of strengths, links differential gene expression to differences in enhancer activity, and creates a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantify enhancer activity in other eukaryotes, including humans.Keywords
This publication has 46 references indexed in Scilit:
- Defining the Status of RNA Polymerase at PromotersCell Reports, 2012
- An integrated encyclopedia of DNA elements in the human genomeNature, 2012
- Transcriptional Enhancers in Animal Development and EvolutionCurrent Biology, 2010
- Phenotypic robustness conferred by apparently redundant transcriptional enhancersNature, 2010
- High-Resolution Mapping and Characterization of Open Chromatin across the GenomeCell, 2008
- Discovery of functional elements in 12 Drosophila genomes using evolutionary signaturesNature, 2007
- Using FlyAtlas to identify better Drosophila melanogaster models of human diseaseNature Genetics, 2007
- Distinct and predictive chromatin signatures of transcriptional promoters and enhancers in the human genomeNature Genetics, 2007
- Polycomb complexes repress developmental regulators in murine embryonic stem cellsNature, 2006
- Expression of a β-globin gene is enhanced by remote SV40 DNA sequencesCell, 1981