Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor
Open Access
- 1 January 2004
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (1), 152-158
- https://doi.org/10.1128/aem.70.1.152-158.2004
Abstract
The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 × 104 CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross-reactivity tests were performed with Escherichia coli and Campylobacter jejuni. To determine whether the MAAB has potential as a screening tool for the diagnosis of asymptomatic Salmonella infection of poultry, chicken excretal samples from a private, noncommercial farm and from university poultry facilities were tested. While the private farm excreta gave rise to signals significantly above the buffer blanks, none of the university samples tested positive for S. enterica serovar Typhimurium without spiking; dose-response curves of spiked excretal samples from university-raised poultry gave limits of detection of 8 × 103 CFU/g.Keywords
This publication has 67 references indexed in Scilit:
- Observations of the relationship between the surface area and weight of eviscerated carcases of chickens, ducks and turkeysInternational Journal of Food Science & Technology, 2007
- Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in PoultryApplied and Environmental Microbiology, 2003
- Piezoelectric Flow Injection Analysis Biosensor for the Detection of Salmonella TyphimuriumJournal of Food Science, 2002
- Detection of Salmonellae in Chicken Feces by a Combination of Tetrathionate Broth Enrichment, Capillary PCR, and Capillary Gel ElectrophoresisJournal of Clinical Microbiology, 2001
- Array biosensor for detection of biohazardsBiosensors and Bioelectronics, 2000
- Food-Related Illness and Death in the United StatesEmerging Infectious Diseases, 1999
- Adsorption of endogenous polyphenols relieves the inhibition by fruit juices and fresh produce of immuno-PCR detection ofEscherichia coli0157:H7FEMS Immunology & Medical Microbiology, 1999
- A compact fiber-optic immunosensor for Salmonella based on evanescent wave excitationSensors and Actuators B: Chemical, 1997
- Patterned planar array immunosensor for multianalyte detectionJournal of Biomedical Optics, 1997
- The direct application of the polymerase chain reaction to DNA extracted from foodsLetters in Applied Microbiology, 1995