The Traditional Medicinal Plants Cuphea calophylla, Tibouchina kingii, and Pseudelephantopus spiralis Attenuate Inflammatory and Oxidative Mediators
Open Access
- 24 April 2018
- journal article
- research article
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 2018, 1-11
- https://doi.org/10.1155/2018/1953726
Abstract
Aerial parts of Cuphea calophylla, Tibouchina kingii, and Pseudelephantopus spiralis have been used in Colombian traditional medicine for inflammation. However, the underlying mechanisms that could explain the anti-inflammatory actions remain unknown. This study aimed to elucidate the anti-inflammatory and cytoprotective effects of hydroalcoholic extracts from C. calophylla (HECC), T. kingii (HETK), and P. spiralis (HEPS) in LPS-stimulated THP-1 macrophages. Reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA) were monitored as inflammatory and oxidative markers. The inhibition of lipoxygenase (LOX) and cyclooxygenase (COX) activities in a cell-free system were also investigated. Antioxidant activities were determined using standard in vitro methods. All extracts inhibited the NO, ROS, and MDA levels. HETK showed the highest ROS production inhibition and the highest antioxidant values, whereas HETK and HEPS significantly decreased the cytotoxicity mediated by LPS. The release of MDA was reduced significantly by all extracts. Moreover, the catalytic activity of LOX was inhibited by HECC and HETK. HECC was a more potent reducer of COX-2 activity. All extracts effectively suppressed COX-1 activity. In summary, these results suggest that HECC, HEPS, and HETK possess anti-inflammatory properties. Therefore, these plants could provide a valuable source of natural bioactive compounds for the treatment of inflammatory-related diseases.Keywords
Funding Information
- Universidad de Antioquia (CPT-1412)
This publication has 54 references indexed in Scilit:
- Macrophage-Mediated Inflammatory DisordersMediators of Inflammation, 2013
- Low-Density Lipoprotein (LDL)-Antioxidant Biflavonoids from Garcinia madrunoMolecules, 2013
- Regulation of the arachidonic acid mobilization in macrophages by combustion-derived particlesParticle and Fibre Toxicology, 2011
- A review on the oxidative and nitrosative stress (O&NS) pathways in major depression and their possible contribution to the (neuro)degenerative processes in that illnessProgress in Neuro-Psychopharmacology and Biological Psychiatry, 2011
- Functional and pathological roles of the 12- and 15-lipoxygenasesProgress in Lipid Research, 2011
- Systematic review of herbals as potential anti-inflammatory agents: Recent advances, current clinical status and future perspectivesPharmacognosy Reviews, 2011
- Omega-3 Fatty Acids and Inflammatory ProcessesNutrients, 2010
- Free radicals and antioxidants in normal physiological functions and human diseaseThe International Journal of Biochemistry & Cell Biology, 2007
- Aldose reductase mediates endotoxin-induced production of nitric oxide and cytotoxicity in murine macrophagesFree Radical Biology & Medicine, 2007
- The Chemistry behind Antioxidant Capacity AssaysJournal of Agricultural and Food Chemistry, 2005