Influence of Temperature, Medium, and Storage Duration on Chlamydia trachomatis DNA Detection by PCR
Open Access
- 1 March 2013
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 51 (3), 990-992
- https://doi.org/10.1128/jcm.02631-12
Abstract
We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.Keywords
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