Injection of 1,25-(OH)2 vitamin D3 enhances resistance artery contractile properties.
- 1 November 1990
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Hypertension
- Vol. 16 (5), 523-531
- https://doi.org/10.1161/01.hyp.16.5.523
Abstract
The hypothesis that 1,25-dihydroxyvitamin D3 [1,25-(OH)2 vitamin D3] modulates vascular smooth muscle contractile function was tested. 1,25-(OH)2 vitamin D3 (50 ng/day) was administered by intraperitoneal injection over a 3-day period to 13-15-week-old male spontaneously hypertensive and Wistar-Kyoto normotensive rats. On the fourth day, serum was prepared and contractile force generation of isolated mesenteric resistance arteries was examined. Treatment with 1,25-(OH)2 vitamin D3 approximately doubled serum levels of the hormone and increased ionized and total serum Ca2+ and phosphate by 5-10%. No effect on blood pressure was detected. 1,25-(OH)2 vitamin D3 injection in both strains enhanced maximal stress generation to norepinephrine and serotonin by 30-40%, with no effect on apparent sensitivity of the vessels to the agonists. To assess the effect of a maneuver that elevates serum ionized Ca2+ without the addition of exogenous hormone, maximal stress generation was examined in resistance arteries isolated from rats fed diets containing 0.5% or 2% calcium over a 6-7-week period. Maximal stress generation in response to norepinephrine was greater in vessels from rats of both strains maintained on 0.5% calcium. It is concluded that 72-hour in vivo treatment with 1,25-(OH)2 vitamin D3 increases contractile force-generating capacity of resistance arteries without affecting blood pressure. It is proposed that this action of 1,25-(OH)2 vitamin D3 is the result of a direct action of the hormone on the vascular wall.Keywords
This publication has 29 references indexed in Scilit:
- Abnormal calcium handling by platelets of spontaneously hypertensive rats.Hypertension, 1990
- 1,25-dihydroxyvitamin D3 stimulates 45Ca2+-uptake by cultured vascular smooth muscle cells derived from rat aortaBiochemical and Biophysical Research Communications, 1988
- 1,25-dihydroxyvitamin D3 binds specifically to rat vascular smooth muscle cells and stimulates their proliferation in vitroLife Sciences, 1988
- Effect of 1,25(OH)2 vitamin D3 and ionized Ca2+ on 45Ca uptake by primary cultures of aortic myocytes of spontaneously hypertensive and Wistar Kyoto normotensive ratsBiochemical and Biophysical Research Communications, 1987
- Receptor for 1,25-dihydroxyvitamin D in a vascular smooth muscle cell line derived from rat aortaBiochemical and Biophysical Research Communications, 1987
- Effects of dihydropyridines on tension and calcium-45 influx in isolated mesenteric resistance vessels from spontaneously hypertensive and normotensive ratsThe American Journal of Cardiology, 1987
- Endothelium-dependent contractions to acetylcholine in the aorta of the spontaneously hypertensive rat.Hypertension, 1986
- Disturbances of calcium metabolism in the spontaneously hypertensive rat.Hypertension, 1981
- Adrenergic neurotransmission in vascular smooth muscle from spontaneously hypertensive rats.Hypertension, 1981
- Changes in noradrenaline sensitivity and morphology of arterial resistance vessels during development of high blood pressure in spontaneously hypertensive rats.Hypertension, 1980