Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing
Open Access
- 2 November 2014
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 12 (3), 230-232
- https://doi.org/10.1038/nmeth.3152
Abstract
Results show the minimum sequencing depth required for the discovery of differentially methylated regions at desired sensitivity and specificity and the trade-off between adding more replicates versus increasing sequencing depth. Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.This publication has 15 references indexed in Scilit:
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