Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites
- 13 December 2004
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 72A (2), 127-135
- https://doi.org/10.1002/jbm.a.30146
Abstract
Hydroxyapatite/soluble calcium phosphate composites (HAp/SCaP) are novel HAp‐based materials with enhanced solubility that have been developed by annealing HAp in a vacuum. This study compared the effects of HAp and HAp/SCaP on osteoblast proliferation, differentiation, and mineralization using an MC3T3‐E1 cell culture system. MC3T3‐E1 cells were cultured on HAp or HAp/SCaP, and the number of attached cells and their morphology were examined. The influence of the extract from HAp/SCaP on osteoblast differentiation was determined by the measurement of alkaline phosphatase activity and reverse transcriptase‐polymerase chain reaction analysis of the expression of osteoblastic markers. In addition, mineralization was evaluated by the staining of calcium deposits with Alizarin red. Attachment of a greater number of cells exhibiting no degeneration in their morphology was observed on HAp/SCaP compared with HAp after incubation for 7 days or more. Culturing cells with the extract from HAp/SCaP resulted in promotion of alkaline phosphatase activity, the expression of type I collagen, and bone‐like tissue formation. The results of the present study indicate that HAp/SCaP shows greater ability in osteogenesis than HAp by increasing collagen synthesis and calcification of the extracellular matrix. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 72A: 127–135, 2005Keywords
This publication has 28 references indexed in Scilit:
- Change in Microstructure and Solubility Improvement of HAp Ceramics by Heat-Treatment in a VacuumMATERIALS TRANSACTIONS, 2002
- Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3‐E1 osteoblast‐like cellsJournal of Orthopaedic Research, 1999
- Characterization of the stromal osteogenic cell line MN7: mRNA steady-state level of selected osteogenic markers depends on cell density and is influenced by 17β-estradiolJournal of Bone and Mineral Research, 1994
- Pattern of gene expression following rat tibial marrow ablationJournal of Bone and Mineral Research, 1993
- Studies of the solubility of different calcium phosphate ceramic particles in vitroBiomaterials, 1990
- Preliminary studies on the phenomenological behaviour of osteoblasts cultured on hydroxyapatite ceramicsBiomaterials, 1990
- Further analysis of spontaneous membrane potential activity and the hyperpolarizing response to parathyroid hormone in osteoblastlike cellsJournal of Cellular Physiology, 1987
- Biodegradation behavior of various calcium phosphate materials in bone tissueJournal of Biomedical Materials Research, 1983
- Calcium Phosphate Ceramics as Hard Tissue ProstheticsClinical Orthopaedics and Related Research, 1981
- Bioceramics consisting of calcium phosphate saltsBiomaterials, 1980