Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry
- 1 April 1998
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 16 (4), 381-384
- https://doi.org/10.1038/nbt0498-381
Abstract
Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.Keywords
This publication has 30 references indexed in Scilit:
- Laser desorption mass spectrometry for point mutation detectionGenetic Analysis: Biomolecular Engineering, 1996
- Detection of hepatitis B virus DNA in serum samples via nested PCR and MALDI-TOF mass spectrometryGenetic Analysis: Biomolecular Engineering, 1996
- A strategy for rapid and efficient DNA sequencing by mass spectrometryNature Biotechnology, 1996
- CLINICAL IMPLICATIONS OF THE p53 GENEAnnual Review of Medicine, 1996
- Efficient preparation of short DNA sequence ladders potentially suitable for MALDI-TOF DNA sequencingGenetic Analysis: Biomolecular Engineering, 1996
- High-Resolution Matrix-Assisted Laser Desorption/Ionization in a Linear Time-of-Flight Mass SpectrometerAnalytical Chemistry, 1995
- Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1995
- Matrix‐assisted laser desorption time‐of‐flight mass spectrometry of oligonucleotides using 3‐hydroxypicolinic acid as an ultraviolet‐sensitive matrixRapid Communications in Mass Spectrometry, 1993
- Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometryRapid Communications in Mass Spectrometry, 1990
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988