ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells
Open Access
- 1 January 2015
- journal article
- research article
- Published by Hindawi Limited in BioMed Research International
- Vol. 2015, 1-9
- https://doi.org/10.1155/2015/423287
Abstract
Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was testedin vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (≥0.01 μg/mL) significantly inhibited proliferation () and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30–40% corresponding to significant depletion (approximately 70–80%) in GSH content in LTEP-a-2 cells (), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.
Funding Information
- Chinese Academy of Sciences (2012017)
This publication has 41 references indexed in Scilit:
- Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular CarcinomaPLOS ONE, 2013
- Zinc Oxide Nanoparticles Induce Necrosis and Apoptosis in Macrophages in a p47phox- and Nrf2-Independent MannerPLOS ONE, 2013
- Induction of reactive oxygen species generation inhibits epithelial–mesenchymal transition and promotes growth arrest in prostate cancer cellsMolecular Carcinogenesis, 2013
- Oxidative stress-induced cytotoxic and genotoxic effects of nano-sized titanium dioxide particles in human HaCaT keratinocytesToxicology, 2012
- Identification of the mechanisms that drive the toxicity of TiO2 particulates: the contribution of physicochemical characteristicsParticle and Fibre Toxicology, 2009
- Preferential killing of cancer cells and activated human T cells using ZnO nanoparticlesNanotechnology, 2008
- Does nanoparticle activity depend upon size and crystal phase?Nanotoxicology, 2008
- Enhancing effects of ceramide derivatives on 1,25-dihydroxyvitamin D3-induced differentiation of human HL-60 leukemia cellsLife Sciences, 2007
- Ambient particle inhalation and the cardiovascular system: potential mechanisms.Environmental Health Perspectives, 2001
- Trypan Blue Exclusion Test of Cell ViabilityCurrent Protocols in Immunology, 1997