Is sperm DNA fragmentation a good marker for field AI bull fertility?1
- 1 August 2012
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Animal Science
- Vol. 90 (8), 2437-2449
- https://doi.org/10.2527/jas.2011-4492
Abstract
This paper aimed at investigating the potential use of sperm DNA fragmentation (SDF) to improve the routine screening of infertility of Holstein bulls. Cryopreserved sperm samples from 201 Holstein bulls provided by an AI center were used in the analyses of SDF at 0 (SDF_0) and 6 (SDF_6) h of incubation at 37°C. A refinement of the sperm chromatin dispersion test implemented in the Sperm-Halomax kit was employed to measure SDF. Records on routinely collected semen traits (volume, concentration, mass and individual motility evaluated in the fresh ejaculate, and individual motility in post-thawed semen straws) were provided by the AI center. Artificial insemination bull fertility was obtained from official field recording as successful or failed insemination. The results show that the average SDF was low (around 3.5%) at 0 and 6 h of incubation. A moderate effect of inbreeding depression was found. Estimated heritability for SDF traits were moderately high (0.41 and 0.29 for SDF_0 and SDF_6, respectively) and estimated repeatability of SDF measures in the same animal were high (0.73 and 0.70 for SDF_0 and SDF_6, respectively). An overall estimated service bull value (ESBV) obtained through statistical modeling that allowed for adjustment of systematic environmental effects not specific to a bull and of the female contribution to fertility, and the estimated genetic values (EGV) were obtained from field-recorded AI information. The ESBV and EGV were also obtained for all semen traits. Moderately large and negative Pearson correlation coefficients were observed between SDF traits and male fertility ranging from (-0.43 to -0.50; P 2 (0.15 to 0.26) among all semen characteristics. Overall, the selected semen traits explained 25% and 31% of the observed variability in bull fertility measured as EGV and ESBV, respectively. When looking at the predictive ability of bull fertility categories, the results of discriminant and logistic regression analyses showed that low-fertility bulls (those in the 10th or lower percentile in the fertility distribution) can be accurately identified by using measures of SDF alone or in combination with sperm motility. Values of SDF around 7% to 10% could be used as indicators of low AI success.Keywords
This publication has 47 references indexed in Scilit:
- Elevated dietary intake of Zn-methionate is associated with increased sperm DNA fragmentation in the boarReproductive Toxicology, 2011
- Analysis of sperm nuclear protein gene polymorphisms and DNA integrity in infertile menSystems Biology in Reproductive Medicine, 2011
- Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human spermFertility and Sterility, 2011
- High Levels of DNA Fragmentation in Spermatozoa Are Associated with Inbreeding and Poor Sperm Quality in Endangered Ungulates1Biology of Reproduction, 2010
- DNA Fragmentation Dynamics in Fresh Versus Frozen Thawed Plus Gradient-Isolated Human SpermatozoaSystems Biology in Reproductive Medicine, 2010
- Is sperm evaluation useful in predicting human fertility?Reproduction, 2007
- DNA Integrity Is Compromised in Protamine‐Deficient Human SpermJournal of Andrology, 2005
- Flow cytometric assessment of trophosphamide toxicity on mouse spermatogenesisCytometry, 1996
- Heterogeneity of Sperm Nuclear Chromatin Structure and its Relationship to Bull Fertility1Biology of Reproduction, 1987
- Relation of Mammalian Sperm Chromatin Heterogeneity to FertilityScience, 1980