A Simplified Method for the Reconstruction of Fully Competent Mouse Zygotes from Adult Somatic Donor Nuclei
- 1 March 2000
- journal article
- research article
- Published by Mary Ann Liebert Inc in Cloning
- Vol. 2 (1), 35-44
- https://doi.org/10.1089/15204550050145111
Abstract
Until now, full-term development of mouse embryos reconstructed from somatic nuclei has been convincingly achieved only when a piezoimpact pipette drive unit is used for the injection of a donor nucleus into an enucleated recipient oocyte. Here we describe a simplified method for mouse cloning that requires neither electrofusion nor a piezo device. Efficient rates of enucleation can be achieved without staining the chromosomes of the recipient oocyte and high survival rates are obtained after direct injection of the donor nucleus. Although a low proportion of reconstructed embryos could implant after their transfer into the oviducts of foster mothers (less than 5%), we show that at least some of them can develop into normal young.Keywords
This publication has 27 references indexed in Scilit:
- Generation of mice from wild-type and targeted ES cells by nuclear cloningNature Genetics, 2000
- Nuclear transfer into mouse zygotesNature Genetics, 2000
- Dynamic Imaging of the Metaphase II Spindle and Maternal Chromosomesin Bovine Oocytes: Implications for Enucleation Efficiency Verification, Avoidanceof Parthenogenesis, and Successful Embryogenesis1Biology of Reproduction, 2000
- The effect of recipient oocyte volume on nuclear transfer in cattleMolecular Reproduction and Development, 1998
- Dynamics of chromatin changes in live one-cell mouse embryos: A continuous follow-up by fluorescence microscopyExperimental Cell Research, 1989
- Differential sensitivity of mouse pronuclei and zygote cytoplasm to Hoechst staining and ultraviolet irradiationReproduction, 1988
- Nuclear transplantation in sheep embryosNature, 1986
- A simple method for counting nuclei in the preimplantation mouse embryoCellular and Molecular Life Sciences, 1985
- Nuclear transplantation in mus musculus: Developmental potential of nuclei from preimplantation embryosCell, 1981
- Nuclear transplantation in the rabbit eggNature, 1975