Volumetric real-time multispectral optoacoustic tomography of biomarkers
- 7 July 2011
- journal article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 6 (8), 1121-1129
- https://doi.org/10.1038/nprot.2011.351
Abstract
Multispectral optoacoustic tomography (MSOT) has recently been developed to enable visualization of optical contrast and tissue biomarkers, with resolution and speed representative of ultrasound. In the implementation described here, MSOT enables operation in real-time mode by capturing single cross-sectional images in <1 ms from living small animals (e.g., mice) and other tissues of similar dimensions. At the core of the method is illumination of the object using multiple wavelengths in order to resolve spectrally distinct biomarkers over background tissue chromophores. The system allows horizontal placement of a mouse in the imaging chamber and three-dimensional scanning of the entire body without the need to immerse the mouse in water. Here we provide a detailed description of the MSOT scanner components, system calibration, selection of image reconstruction algorithms and animal handling. Overall, the entire protocol can be completed within 15-30 min for acquisition of a whole-body multispectral data set from a living mouse.Keywords
This publication has 43 references indexed in Scilit:
- Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomographyOptics Express, 2010
- Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopyNeuroImage, 2010
- Real-time photoacoustic tomography of cortical hemodynamics in small animalsJournal of Biomedical Optics, 2010
- Whole-body three-dimensional optoacoustic tomography system for small animalsJournal of Biomedical Optics, 2009
- Functional transcranial brain imaging by optical-resolution photoacoustic microscopyJournal of Biomedical Optics, 2009
- Imaging of mesoscopic-scale organisms using selective-plane optoacoustic tomographyPhysics in Medicine & Biology, 2009
- Sensitivity of molecular target detection by multispectral optoacoustic tomography (MSOT)Medical Physics, 2009
- Realtime Photoacoustic Microscopy of Murine Cardiovascular DynamicsOptics Express, 2008
- Monkey brain cortex imaging by photoacoustic tomographyJournal of Biomedical Optics, 2008
- In vivo imaging of subcutaneous structures using functional photoacoustic microscopyNature Protocols, 2007