Overexpression of cytoplasmic dynein's globular head causes a collapse of the interphase microtubule network in Dictyostelium.
Open Access
- 1 June 1996
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 7 (6), 935-948
- https://doi.org/10.1091/mbc.7.6.935
Abstract
Cytoplasmic dynein is a minus-end directed microtubule-based motor. Using a molecular genetic approach, we have begun to dissect structure-function relationships of dynein in the cellular slime mold Dictyostelium. Expression of a carboxy-terminal 380-kDa fragment of the heavy chain produces a protein that approximates the size and shape of the globular, mechanochemical head of dynein. This polypeptide cosediments with microtubules in an ATP-sensitive fashion and undergoes a UV-vanadate cleavage reaction. The deleted amino-terminal region appears to participate in dimerization of the native protein and in binding the intermediate and light chains. Overexpression of the 380-kDa carboxy-terminal construct in Dictyostelium produces a distinct phenotype in which the interphase radial microtubule array appears collapsed. In many cells, the microtubules form loose bundles that are whorled around the nucleus. Similar expression of a central 107-kDa fragment of the heavy chain does not produce this result. The data presented here suggest that dynein may participate in maintaining the spatial pattern of the interphase microtubule network.Keywords
This publication has 45 references indexed in Scilit:
- Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.The Journal of cell biology, 1995
- Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles.The Journal of cell biology, 1994
- Cytoplasmic dynein undergoes intracellular redistribution concomitant with phosphorylation of the heavy chain in response to serum starvation and okadaic acid.The Journal of cell biology, 1994
- A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chainThe Journal of cell biology, 1993
- ATP-insensitive interaction of the amino-terminal region of the .beta. heavy chain of dynein with microtubulesBiochemistry, 1993
- Mechanism of centrosome positioning during the wound response in BSC-1 cells.The Journal of cell biology, 1992
- Microsurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cellsCell, 1991
- Quantification of transformation efficiency using a new method for clonal growth and selection of axenic Dictyostelium cellsDevelopmental Genetics, 1990
- Micromanipulation studies of the asyrnmetric positioning of the maturation spindle in Chaetopterus sp. Oocytes: I. Anchorage of the spindle to the cortex and migration of a displaced spindleCell Motility, 1988
- Beyond self-assembly: From microtubules to morphogenesisCell, 1986