Coupling endoplasmic reticulum stress to the cell death program in mouse melanoma cells: effect of curcumin
- 21 May 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Apoptosis
- Vol. 13 (7), 904-914
- https://doi.org/10.1007/s10495-008-0221-x
Abstract
The microenvironment of cancerous cells includes endoplasmic reticulum (ER) stress the resistance to which is required for the survival and growth of tumors. Acute ER stress triggers the induction of a family of ER stress proteins that promotes survival and/or growth of the cancer cells, and also confers resistance to radiation and chemotherapy. Prolonged or severe ER stress, however, may ultimately overwhelm the cellular protective mechanisms, triggering cell death through specific programmed cell death (pcd) pathways. Thus, downregulation of the protective stress proteins may offer a new therapeutic approach to cancer treatment. In this regard, recent reports have demonstrated the roles of the phytochemical curcumin in the inhibition of proteasomal activity and triggering the accumulation of cytosolic Ca2+ by inhibiting the Ca2+-ATPase pump, both of which enhance ER stress. Using a mouse melanoma cell line, we investigated the possibility that curcumin may trigger ER stress leading to programmed cell death. Our studies demonstrate that curcumin triggers ER stress and the activation of specific cell death pathways that feature caspase cleavage and activation, p23 cleavage, and downregulation of the anti-apoptotic Mcl-1 protein.Keywords
This publication has 69 references indexed in Scilit:
- Evidence for Involvement of BH3-Only Proapoptotic Members in Adenovirus-Induced ApoptosisJournal of Virology, 2007
- Endoplasmic reticulum stress-induced cell death mediated by the proteasomeCell Death & Differentiation, 2007
- Curcumin induces pro-apoptotic endoplasmic reticulum stress in human leukemia HL-60 cellsBiochemical and Biophysical Research Communications, 2007
- Endoplasmic Reticulum Stress Inhibition Protects against Excitotoxic Neuronal Injury in the Rat BrainJournal of Neuroscience, 2007
- CANCER CHEMOPREVENTIVE EFFECTS OF CURCUMINAdvances in experimental medicine and biology, 2006
- CURCUMIN: THE INDIAN SOLID GOLDAdvances in experimental medicine and biology, 2006
- Expression of Mcl-1 in cerebellar granule neurons is regulated by IGF-I in a developmentally specific fashionDevelopmental Brain Research, 2004
- BAX and BAK Regulation of Endoplasmic Reticulum Ca 2+ : A Control Point for ApoptosisScience, 2003
- Coupling Endoplasmic Reticulum Stress to the Cell Death ProgramOnline Journal of Public Health Informatics, 2001
- Inhibitory effects of topical application of low doses of curcumin on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion and oxidized DNA bases in mouse epidermisCarcinogenesis: Integrative Cancer Research, 1997