A positive relationship between protein synthetic rate and intracellular glutamine concentration in perfused rat skeletal muscle
- 4 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 215 (1), 187-191
- https://doi.org/10.1016/0014-5793(87)80139-4
Abstract
During muscle-protein wasting associated with injury and disease the distribution ratio of free glutamine between muscle and blood falls. In pursuing possible consequences of this, we investigated the relationship between the rate of muscle protein synthesis and intramuscular glutamine concentration, manipulated acutely in the isolated perfused rat hindquarter. Increasing perfusate glutamine from 0.67 to 5.0 mM caused a 200% increase in intracellular glutamine and a 66% increase in protein synthesis in the absence of insulin; in the presence of insulin a 30% increase in intramuscular glutamine was accompanied by an 80% increase in protein synthesis. Analysis of variance of the results confirmed the existence of positive relationships between intramuscular glutamine and protein synthesis in the presence or absence of insulin. Control of the size of the intramuscular free pool of glutamine may be important in determining the muscle protein mass.This publication has 12 references indexed in Scilit:
- Differential effects of live bacteria and lipopolysaccharide on amino acid and 2-deoxy-d-glucose transport in rat muscleBiochemical Society Transactions, 1986
- Amino acid transport in perfused rat skeletal muscleBiochemical Society Transactions, 1986
- To what extent do kinetic properties of amino acid transporters in liver dictate the net exchange of amino acids in vivo?Biochemical Society Transactions, 1986
- CHARACTERISTICS OF A GLUTAMINE CARRIER IN SKELETAL MUSCLE HAVE IMPORTANT CONSEQUENCES FOR NITROGEN LOSS IN INJURY, INFECTION, AND CHRONIC DISEASEThe Lancet, 1986
- Therapeutic Aspects of Branched-Chain Amino and Keto AcidsClinical Science, 1984
- The regulation of neutral amino acid transport in mammalian cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1983
- Regulation by insulin of amino acid release and protein turnover in the perfused rat hemicorpus.Online Journal of Public Health Informatics, 1977
- Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolismBiochemical Journal, 1971
- Regulatory significance of transfer RNA charging levels I. Measurements of charging levels in livers of chow-fed rats, fasting rats, and rats fed balanced or imbalanced mixtures of amino acidsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTOnline Journal of Public Health Informatics, 1951