Direct analysis of selected N‐acyl‐L‐homoserine lactones by gas chromatography/mass spectrometry
- 14 May 2004
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 18 (12), 1341-1344
- https://doi.org/10.1002/rcm.1480
Abstract
A rapid, simple and selective method involving direct separation by gas chromatography (GC) with electron ionization mass spectrometry (EI-MS) was employed to determine some N-acylhomoserine lactones (AHLs). Using GC/EI-MS, simultaneous separation and characterization of AHLs were possible without prior derivatization. Informative fragmentation patterns were obtained to identify the structures of N-acyl chains of AHLs. Electron ionization resulted in a common fragmentation pattern with the most abundant ion at m/z 143 and other minor peaks at m/z 71, 57, and 43. The presence of AHLs in extracts of Burkholderia cepacia strains was achieved in selected ion monitoring mode by using the prominent fragment at m/z 143. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
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