Light‐induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis
Open Access
- 14 January 2005
- journal article
- Published by Wiley in Plant, Cell & Environment
- Vol. 28 (3), 340-352
- https://doi.org/10.1111/j.1365-3040.2005.01270.x
Abstract
Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non‐invasively using ion‐selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non‐growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 µmol m−2 s−1) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light‐induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light‐induced K+ flux changes are likely to play a charge balancing role. Light‐stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light‐induced ion flux changes are associated with both leaf growth and photosynthesis.Keywords
This publication has 57 references indexed in Scilit:
- Blue-Light Photoreceptors in Higher PlantsAnnual Review of Cell and Developmental Biology, 1999
- Calcium-calmodulin signalling is involved in light-induced acidification by epidermal leaf cells of pea, Pisum sativum LJournal of Experimental Botany, 1997
- CO2‐concentrating mechanisms: a direct role for thylakoid lumen acidification?Plant, Cell & Environment, 1997
- Red and blue light-stimulated proton efflux by epidermal leaf cells of the Argenteum mutant ofPisum sativumJournal of Experimental Botany, 1994
- Stimulation of Growth and Ion Uptake in Bean Leaves by Red and Blue LightPlant Physiology, 1992
- Growth and Deposition of Inorganic Nutrient Elements in Developing Leaves of Zea mays L.Plant Physiology, 1992
- Structure and function of proton translocating ATPase in plasma membranes of plants and fungiBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1988
- The Control Of Leaf ExpansionAnnual Review of Plant Physiology and Plant Molecular Biology, 1988
- Low Proton Conductance of Plant Cuticles and Its Relevance to the Acid-Growth TheoryPlant Physiology, 1981
- Photosynthesis and apparent proton fluxes in Elodea canadensisZeitschrift für Pflanzenphysiologie, 1972