Three-Dimensional Cell Culture Matrices: State of the Art
Top Cited Papers
- 1 March 2008
- journal article
- review article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part B: Reviews
- Vol. 14 (1), 61-86
- https://doi.org/10.1089/teb.2007.0150
Abstract
Traditional methods of cell growth and manipulation on 2-dimensional (2D) surfaces have been shown to be insufficient for new challenges of cell biology and biochemistry, as well as in pharmaceutical assays. Advances in materials chemistry, materials fabrication and processing technologies, and developmental biology have led to the design of 3D cell culture matrices that better represent the geometry, chemistry, and signaling environment of natural extracellular matrix. In this review, we present the status of state-of-the-art 3D cell-growth techniques and scaffolds and analyze them from the perspective of materials properties, manufacturing, and functionality. Particular emphasis was placed on tissue engineering and in vitro modeling of human organs, where we see exceptionally strong potential for 3D scaffolds and cell-growth methods. We also outline key challenges in this field and most likely directions for future development of 3D cell culture over the period of 5–10 years.Keywords
This publication has 189 references indexed in Scilit:
- Physical properties of alginate hydrogels and their effects on in vitro follicle developmentBiomaterials, 2007
- Fabrication of porous ultra-short single-walled carbon nanotube nanocomposite scaffolds for bone tissue engineeringBiomaterials, 2007
- Progress and opportunities for tissue-engineered skinNature, 2007
- Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic CulturesScience, 2006
- Tissue-Engineered Follicles Produce Live, Fertile OffspringTissue Engineering, 2006
- Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysisProceedings of the National Academy of Sciences, 2006
- Controlling interlayer diffusion to achieve sustained, multiagent delivery from layer-by-layer thin filmsProceedings of the National Academy of Sciences, 2006
- Expansion of engrafting human hematopoietic stem/progenitor cells in three‐dimensional scaffolds with surface‐immobilized fibronectinJournal of Biomedical Materials Research Part A, 2006
- Inductive tissue engineering with protein and DNA-releasing scaffoldsMolecular BioSystems, 2005
- Presentation and Recognition of Biotin on Nanofibers Formed by Branched Peptide AmphiphilesNano Letters, 2004