Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells
- 19 March 2012
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 109 (14), 5394-5398
- https://doi.org/10.1073/pnas.1121632109
Abstract
The question of whether a single hematopoietic stem cell (HSC) gives rise to all of the B-cell subsets [B-1a, B-1b, B-2, and marginal zone (MZ) B cells] in the mouse has been discussed for many years without resolution. Studies here finally demonstrate that individual HSCs sorted from adult bone marrow and transferred to lethally irradiated recipients clearly give rise to B-2, MZ B, and B-1b, but does not detectably reconstitute B-1a cells. These findings place B-2, MZ, and B-1b in a single adult developmental lineage and place B-1a in a separate lineage derived from HSCs that are rare or missing in adults. We discuss these findings with respect to known developmental heterogeneity in other HSC-derived lymphoid, myeloid, and erythroid lineages, and how HSC developmental heterogeneity conforms to the layered model of the evolution of the immune system that we proposed some years ago. In addition, of importance to contemporary medicine, we consider the implications that HSC developmental heterogeneity may have for selecting HSC sources for human transplantation.Keywords
This publication has 36 references indexed in Scilit:
- Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcodingNature Biotechnology, 2011
- Reduced production of B-1–specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cellsProceedings of the National Academy of Sciences of the United States of America, 2011
- Distinct progenitors for B-1 and B-2 cells are present in adult mouse spleenProceedings of the National Academy of Sciences of the United States of America, 2011
- Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−The Journal of Experimental Medicine, 2011
- Embryonic day 9 yolk sac and intra-embryonic hemogenic endothelium independently generate a B-1 and marginal zone progenitor lacking B-2 potentialProceedings of the National Academy of Sciences of the United States of America, 2011
- Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartmentThe Journal of Experimental Medicine, 2010
- Functionally distinct hematopoietic stem cells modulate hematopoietic lineage potential during aging by a mechanism of clonal expansionProceedings of the National Academy of Sciences of the United States of America, 2010
- Ordering human CD34+CD10−CD19+pre/pro-B-cell and CD19−common lymphoid progenitor stages in two pro-B-cell development pathwaysProceedings of the National Academy of Sciences of the United States of America, 2010
- Sox17 Dependence Distinguishes the Transcriptional Regulation of Fetal from Adult Hematopoietic Stem CellsCell, 2007
- Phenotypically distinct B cell development pathways map to the three B cell lineages in the mouseProceedings of the National Academy of Sciences of the United States of America, 2006