The early stages of taxol biosynthesis: An interim report on the synthesis and identification of early pathway metabolites
- 1 May 2012
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Natural Product Reports
- Vol. 29 (6), 683-696
- https://doi.org/10.1039/c2np20021j
Abstract
Covering: 1966 to 2012 The biosynthesis of the anti-cancer drug taxol (paclitaxel) has required the collaborative efforts of several research groups to tackle the synthesis and labeling of putative biosynthetic intermediates, in concert with the identification, cloning and functional expression of the biosynthetic genes responsible for the construction of this complex natural product. Based on a combination of precursor labeling and incorporation experiments, and metabolite isolation from Taxus spp., a picture of the complex matrix of pathway oxygenation reactions following formation of the first committed intermediate, taxa-4(5),11(12)-diene, is beginning to emerge. An overview of the current state of knowledge on the early-stages of taxol biosynthesis is presented.Keywords
This publication has 84 references indexed in Scilit:
- Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesisNature, 2010
- Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coliScience, 2010
- Synthesis and in vitro evaluation of taxol oxetane ring D precursorsTetrahedron Letters, 2010
- Studies on Taxol® biosynthesis. Preparation and tritium labeling of biosynthetic intermediates by deoxygenation of a taxadiene tetra‐acetate obtained from Japanese yewJournal of Labelled Compounds and Radiopharmaceuticals, 2008
- Expanding the metabolic engineering toolbox: more options to engineer cellsTrends in Biotechnology, 2007
- Cytochrome P450 oxygenases of Taxol biosynthesisPhytochemistry Reviews, 2006
- Preliminary assessment of the C13-side chain 2′-hydroxylase involved in Taxol biosynthesisBiochemical and Biophysical Research Communications, 2005
- Taxol Biosynthesis: Molecular Cloning and Characterization of a Cytochrome P450 Taxoid 7β-HydroxylaseCell Chemical Biology, 2004
- Enantiospecific Total Synthesis of Natural (+)-Taxusin. 2. Functionalization of the A-Ring and Arrival at the TargetJournal of the American Chemical Society, 1998
- TAXANE DITERPENE SYNTHESIS STRATEGIES. A REVIEWOrganic Preparations and Procedures International, 1991