Evidence is presented to show that under certain conditions, three species of Japanese talpine mole, Euroscaptor mizura, Mogera wogura, and Mogera kobeae, persistently use the same nest or nesting site. The moles' nesting sites were detected by the fruiting of an agaric species, Hebeloma radicosum, which specifically colonizes a mole's latrines near its nest. The nests were removed to observe the moles' response. During excavation, the moles often returned to the disturbed sites in search of their nests. The nests were soon reconstructed, followed by refruiting of the mushroom. Thus, one nesting site of E. mizura was used for more than 15 years, despite removal of the nest seven times, and another for more than 5 years, a period ended by the capture of the occupant. Similar results were also obtained with M. wogura and M. kobeae. The occupants may have changed generationally at each site. These persistently used sites seem to be associated with well-drained soil and appropriate vegetation.