Bioavailability study of a polyphenol‐enriched extract from Hibiscus sabdariffa in rats and associated antioxidant status
- 15 August 2012
- journal article
- research article
- Published by Wiley in Molecular Nutrition & Food Research
- Vol. 56 (10), 1590-1595
- https://doi.org/10.1002/mnfr.201200091
Abstract
The aqueous extracts of Hibiscus sabdariffa have been commonly used in folk medicine. Nevertheless, the compounds or metabolites responsible for its healthy effects have not yet been identified. The major metabolites present in rat plasma after acute ingestion of a polyphenol‐enriched Hibiscus sabdariffa extract were characterized and quantified in order to study their bioavailability. The antioxidant status of the plasma samples was also measured through several complementary antioxidant techniques. High‐performance liquid chromatography coupled to time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS) was used for the bioavailability study. The antioxidant status was measured by ferric reducing ability of plasma method, thiobarbituric acid reactive substances assay, and superoxide dismutase activity assay. Seventeen polyphenols and metabolites have been detected and quantified. Eleven of these compounds were metabolites. Although phenolic acids were found in plasma without any modification in their structures, most flavonols were found as quercetin or kaempferol glucuronide conjugates. Flavonol glucuronide conjugates, which show longer half‐life elimination values, are proposed to contribute to the observed lipid peroxidation inhibitory activity in the cellular membranes. By contrast, phenolic acids appear to exert their antioxidant activity through ferric ion reduction and superoxide scavenging at shorter times. We propose that flavonol‐conjugated forms (quercetin and kaempferol) may be the compounds responsible for the observed antioxidant effects and contribute to the healthy effects of H. sabdariffa polyphenolic extract.Keywords
This publication has 16 references indexed in Scilit:
- Pharmacokinetic Profile of Total Quercetin after Single Oral Dose of Tartary Buckwheat Extracts in RatsJournal of Agricultural and Food Chemistry, 2011
- Phenolic Compounds Responsible for the Superoxide Dismutase-like Activity in High-Brix Apple VinegarJournal of Agricultural and Food Chemistry, 2010
- The aqueous extract of Hibiscus sabdariffa calices modulates the production of monocyte chemoattractant protein-1 in humansPhytomedicine, 2010
- Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MSJournal of Separation Science, 2009
- Metabolism, oral bioavailability and pharmacokinetics of chemopreventive kaempferol in ratsBiopharmaceutics & Drug Disposition, 2009
- Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extractFood Chemistry, 2009
- Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers ofHibiscus sabdariffa LMolecular Nutrition & Food Research, 2005
- A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stressNutrition, Metabolism and Cardiovascular Diseases, 2005
- The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP AssayAnalytical Biochemistry, 1996
- Chemical Structure and Biological Activity of Polysaccharides fromHibiscus sabdariffaPlanta Medica, 1992