Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB
- 8 August 2016
- journal article
- Published by Spandidos Publications in Oncology Letters
- Vol. 12 (4), 2840-2845
- https://doi.org/10.3892/ol.2016.4971
Abstract
In the present study, the effects and molecular mechanisms of thymoquinone (TQ) on colon cancer cells were investigated. Cell viability was determined using a Cell Counting Kit‑8 assay, and the results revealed that treatment with TQ significantly decreased cell viability in COLO205 and HCT116 cells in a dose‑dependent manner. TQ treatment additionally sensitized COLO205 and HCT116 cells to cisplatin therapy in a concentration‑dependent manner. To investigate the molecular mechanisms of TQ action, western blot analysis was used to determine the levels of phosphorylated p65 and nuclear factor‑κB (NF‑κB)‑regulated gene products vascular endothelial growth factor (VEGF), c‑Myc and B‑cell lymphoma 2 (Bcl‑2). The results indicated that TQ treatment significantly decreased the level of phosphorylated p65 in the nucleus, which indicated the inhibition of NF‑κB activation by TQ treatment. Treatment with TQ also decreased the expression levels of VEGF, c‑Myc and Bcl‑2. In addition, the inhibition of NF‑κB activation with a specific inhibitor, pyrrolidine dithiocarbamate, potentiated the induction of cell death and caused a chemosensitization effect of TQ in colon cancer cells. Overall, the results of the present study suggested that TQ induced cell death and chemosensitized colon cancer cells by inhibiting NF‑κB signaling.Keywords
This publication has 54 references indexed in Scilit:
- Thymoquinone Inhibits Tumor Growth and Induces Apoptosis in a Breast Cancer Xenograft Mouse Model: The Role of p38 MAPK and ROSPLOS ONE, 2013
- Thymoquinone Induces Mitochondria-Mediated Apoptosis in Acute Lymphoblastic Leukaemia in VitroMolecules, 2013
- Thymoquinone Inhibits Autophagy and Induces Cathepsin-Mediated, Caspase-Independent Cell Death in Glioblastoma CellsPLOS ONE, 2013
- Thymoquinone attenuates tumor growth in ApcMin mice by interference with Wnt-signalingMolecular Cancer, 2013
- Targeted Apoptotic Effects of Thymoquinone and Tamoxifen on XIAP Mediated Akt Regulation in Breast CancerPLOS ONE, 2013
- Chemoprevention in familial adenomatous polyposisBest Practice & Research Clinical Gastroenterology, 2011
- Thymoquinone decreases F-actin polymerization and the proliferation of human multiple myeloma cells by suppressing STAT3 phosphorylation and Bcl2/Bcl-XL expressionLipids in Health and Disease, 2011
- Thymoquinone inhibits proliferation, induces apoptosis and chemosensitizes human multiple myeloma cells through suppression of signal transducer and activator of transcription 3 activation pathwayBritish Journal of Pharmacology, 2010
- Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivoJournal of Experimental & Clinical Cancer Research, 2010
- Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008International Journal of Cancer, 2010