Colony Forming Cell-Fibroblast Development in Extracellular Matrix-Induced Bone and Bone Marrow Formation in Rat

Abstract

The development of murine endochondral bone and bone marrow as a result of demineralized bone matrix implantation is preceded by the accumulation and proliferation of colony forming cell fibroblasts. These cells appear first at 24 hours post-implantation, after which they increase in 2 swells, achieving peak number between days 10-14. The observed differences in developmental kinetics of colony forming cell fibroblasts in culture were not found to be related to qualitative differences in the synthesis of collagens, fibronectin, laminin or proteoglycans. The colony forming cell fibroblasts were shown to be radiosensitive, with the Do = 339 ± 63.