Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms
- 1 June 2004
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 150 (6), 1769-1777
- https://doi.org/10.1099/mic.0.26797-0
Abstract
Fifty strains belonging to Vibrio harveyi, Vibrio campbellii, and the recently described Vibrio rotiferianus, were analysed using phenotypic and genomic techniques with the aim of analysing the usefulness of the different techniques for the identification of V. harveyi-related species. The species V. harveyi and V. campbellii were phenotypically indistinguishable by more than 100 phenotypic features. Thirty-nine experimental strains were phenotypically identified as V. harveyi, but FAFLP, REP-PCR, IGS-PCR and DNA–DNA hybridization proved that they in fact belong to the species V. campbellii. Similar groupings were found among all fingerprinting methodologies (except IGS-PCR). Thirty-two experimental strains clustered with the V. campbellii type and one reference strain; seven strains clustered with the V. harveyi type and three reference strains; and the type and four reference strains of V. rotiferianus grouped together. The correlations between DNA–DNA hybridization and the genomic fingerprinting by FAFLP and (GTG)5-PCR were found to be above 0·68 and statistically significant, suggesting the value of the latter techniques for the reliable identification of V. harveyi-related species. The results presented indicate that strains phenotypically identified as V. harveyi are in fact V. campbellii; these findings position V. campbellii as an important species involved in diseases of reared aquatic organisms.Keywords
This publication has 33 references indexed in Scilit:
- Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus VirgibacillusInternational Journal of Systematic and Evolutionary Microbiology, 2003
- Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornisInternational Journal of Systematic and Evolutionary Microbiology, 2003
- Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilisInternational Journal of Systematic and Evolutionary Microbiology, 2003
- 16S ribosomal DNA sequencing confirms the synonymy of Vibrio harveyi and V. carchariaeDiseases of Aquatic Organisms, 2002
- Applicability of rep-PCR fingerprinting for identification ofLactobacillusspeciesFEMS Microbiology Letters, 2001
- Genomic approaches to typing, taxonomy and evolution of bacterial isolates.International Journal of Systematic and Evolutionary Microbiology, 2001
- Use of Repetitive DNA Sequences and the PCR To Differentiate Escherichia coli Isolates from Human and Animal SourcesApplied and Environmental Microbiology, 2000
- Vibrio penaeicida sp. nov., a Pathogen of Kuruma Prawns (Penaeus japonicus)International Journal of Systematic and Evolutionary Microbiology, 1995
- A set of keys for biochemical identification of environmental Vibrio speciesJournal of Applied Bacteriology, 1994
- Towards a Phylogeny of the Genus Vibrio Based on 16S rRNA SequencesInternational Journal of Systematic and Evolutionary Microbiology, 1992