Three isolation techniques, plating sieved debris, plating root pieces, and soil plating were tested in conjunction with 3 isolation media (Martin''s rose bengal, Komada and Nash-Snyder) for recovery of Fusarium spp. from [grassland] soil. Comparisons among isolation techniques and media were based on recovery of 2057 isolates representing 17 spp. plus 2 varieties of Fusarium. Recovery of individual species of Fusarium was dependent on the type of isolation technique used, but generally was not dependent on the medium used. There were significant interactions between isolation techniques and media. Diversity indices of the Fusarium spp. recovered were highest with the debris technique and with Martin''s rose bengal medium. Determination of the presence and abundance of a Fusarium sp. in soil is dependent on appropriate isolation procedures.