NAD‐sensitive hydrogel for the release of macromolecules
- 20 August 2004
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 87 (6), 791-796
- https://doi.org/10.1002/bit.20210
Abstract
A hydrogel membrane containing immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and hemoglobin). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked to dextran molecules that were subsequently crosslinked to form a gel. The resulting stable hydrogels contained both covalent and affinity crosslinks such that their intrinsic porosities were sensitive to competitive displacers of the affinity interaction between lysozyme and Cibacron blue. Transport experiments in a twin chamber diffusion cell showed that as NAD was added to the donor side, the dissociation of the binding sites between the Cibacron blue and the lysozyme led to an increase in protein diffusion through the hydrogel. The results showed that addition of NAD caused a saturable concentration‐dependent increase in the transport of both cytochrome C and hemoglobin. This effect was shown to be both specific and reversible.Keywords
This publication has 23 references indexed in Scilit:
- Pulsatile drug release control using hydrogelsAdvanced Drug Delivery Reviews, 2001
- Modulated insulin delivery from glucose-sensitive hydrogel dosage formsJournal of Controlled Release, 2001
- Novel pH-sensitive hydrogels with adjustable swelling kineticsBiomaterials, 1998
- Temperature-responsive gels and thermogelling polymer matrices for protein and peptide deliveryAdvanced Drug Delivery Reviews, 1998
- Dynamic Behavior of Glucose-Responsive Poly(methacrylic acid-g-ethylene glycol) HydrogelsMacromolecules, 1997
- A novel drug delivery system utilizing a glucose responsive polymer complex between poly (vinyl alcohol) and poly (N-vinyl-2-pyrrolidone) with a phenylboronic acid moietyJournal of Controlled Release, 1992
- Synthesis and application of thermally reversible heterogels for drug deliveryJournal of Controlled Release, 1990
- An insulin-releasing system that is responsive to glucoseJournal of Controlled Release, 1989
- pH-Dependent Swelling and Solute Diffusion Characteristics of Poly(Hydroxyethyl Methacrylate–CO–Methacrylie Acid) HydrogelsPharmaceutical Research, 1988
- A Glucose-Controlled Insulin-Delivery System: Semisynthetic Insulin Bound to LectinScience, 1979