Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose
Open Access
- 15 April 2011
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (8), 2727-2733
- https://doi.org/10.1128/aem.02454-10
Abstract
Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis. Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism.Keywords
This publication has 32 references indexed in Scilit:
- Development of pyrF- Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta MutantApplied and Environmental Microbiology, 2010
- Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A reviewBioresource Technology, 2010
- Exploring Biodiversity for Cellulosic Biofuel ProductionChemistry & Biodiversity, 2010
- Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol productionBMC Systems Biology, 2010
- Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol ProductionApplied and Environmental Microbiology, 2009
- Expanding metabolism for biosynthesis of nonnatural alcoholsProceedings of the National Academy of Sciences of the United States of America, 2008
- Consolidated Bioprocessing for Bioethanol Production Using Saccharomyces cerevisiaePublished by Springer Science and Business Media LLC ,2007
- Metabolic Engineering of Carotenoid Biosynthesis in Escherichia coli by Ordered Gene Assembly in Bacillus subtilisApplied and Environmental Microbiology, 2007
- Outlook for cellulase improvement: Screening and selection strategiesBiotechnology Advances, 2006
- Construction of a recombinant cellulolytic Escherichia coliGene, 1995