Engineering an in vitro air-blood barrier by 3D bioprinting
Open Access
- 22 January 2015
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 5 (1), srep07974
- https://doi.org/10.1038/srep07974
Abstract
Intensive efforts in recent years to develop and commercialize in vitro alternatives in the field of risk assessment have yielded new promising two- and three dimensional (3D) cell culture models. Nevertheless, a realistic 3D in vitro alveolar model is not available yet. Here we report on the biofabrication of the human air-blood tissue barrier analogue composed of an endothelial cell, basement membrane and epithelial cell layer by using a bioprinting technology. In contrary to the manual method, we demonstrate that this technique enables automatized and reproducible creation of thinner and more homogeneous cell layers, which is required for an optimal air-blood tissue barrier. This bioprinting platform will offer an excellent tool to engineer an advanced 3D lung model for high-throughput screening for safety assessment and drug efficacy testing.This publication has 54 references indexed in Scilit:
- An improved 3D tetraculture system mimicking the cellular organisation at the alveolar barrier to study the potential toxic effects of particles on the lungParticle and Fibre Toxicology, 2013
- Development of a valve-based cell printer for the formation of human embryonic stem cell spheroid aggregatesBiofabrication, 2013
- Biofabrication of Osteochondral Tissue Equivalents by Printing Topologically Defined, Cell-Laden Hydrogel ScaffoldsTissue Engineering, Part C: Methods, 2012
- Biofabrication of a three-dimensional liver micro-organ as an in vitro drug metabolism modelBiofabrication, 2010
- Reduce, Refine, ReplaceNature Immunology, 2010
- Matrigel: A complex protein mixture required for optimal growth of cell cultureProteomics, 2010
- Scaffold-free vascular tissue engineering using bioprintingBiomaterials, 2009
- Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabricationBiomaterials, 2008
- Cell Culture Models of the Respiratory Tract Relevant to Pulmonary Drug DeliveryJournal of Aerosol Medicine, 2005
- The normal human lung: ultrastructure and morphometric estimation of diffusion capacityRespiration Physiology, 1978