Depletion of Intracellular Zinc Inhibits the Ubiquitin Ligase Activity of Viral Regulatory Protein ICP0 and Restricts Herpes Simplex Virus 1 Replication in Cell Culture
Open Access
- 1 April 2012
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (7), 4029-4033
- https://doi.org/10.1128/jvi.06962-11
Abstract
The viral ubiquitin ligase ICP0 stimulates the onset of HSV-1 lytic infection and productive reactivation of viral genomes from latency. In order to mediate these processes, it requires its C3HC4 RING finger domain, a tertiary structural fold that is coordinated by the binding of two zinc (Zn 2+ ) atoms. Here we formally demonstrate that Zn 2+ binding and intracellular Zn 2+ levels are critical for ICP0's biochemical activity and that depletion of intracellular Zn 2+ severely attenuates HSV-1 replication.Keywords
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