PREPARATIVE SEPARATION OF LUVANGETIN FROM ZANTHOXYLUM AILANTHOIDES SIEB. & ZUCC. BY CENTRIFUGAL PARTITION CHROMATOGRAPHY
- 1 April 2014
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Liquid Chromatography & Related Technologies
- Vol. 37 (13), 1819-1826
- https://doi.org/10.1080/10826076.2013.809546
Abstract
□ Luvangetin, a coumarin compound was separated from the extract of stem bark of Zanthoxylum ailanthoides Sieb. & Zucc. by centrifugal partition chromatography (CPC) using a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (2:2:2:1, v/v). Its chemical structural was elucidated by electrospray ionization mass spectrometry,1 H Nuclear Magnetic Resonance (NMR), and 13C NMR. The ethanol extract of the stem bark of Z. ailanthoides was fractioned by extraction with petroleum ether, ethyl acetate, and n-butanol successively. The petroleum ether fraction was subjected to further CPC separation. From 500 mg of petroleum ether extract, 20.6 mg of high pure (99% by high-performance liquid chromatography (HPLC) analysis) luvangetin was obtained in about 30 min.Keywords
Funding Information
- The authors acknowledge financial support of this work by Beijing Natural Science Foundation (No. 2102016).
This publication has 13 references indexed in Scilit:
- Isolation of substances with antiproliferative and apoptosis-inducing activities against leukemia cells from the leaves of Zanthoxylum ailanthoides Sieb. & ZuccPhytomedicine, 2011
- Protective effects of leaf extract of Zanthoxylum ailanthoides on oxidation of low-density lipoprotein and accumulation of lipid in differentiated THP-1 cellsFood and Chemical Toxicology, 2009
- Amides and Benzenoids from Zanthoxylum ailanthoides with Inhibitory Activity on Superoxide Generation and Elastase Release by NeutrophilsJournal of Natural Products, 2009
- Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & zucc.Food Chemistry, 2006
- Two new sesquiterpenoids and anti-HIV principles from the root bark of Zanthoxylum ailanthoidesBioorganic & Medicinal Chemistry, 2005
- Chemical Constituents from the Root Bark of Formosan Zanthoxylum AilanthoidesJournal of the Chinese Chemical Society, 2003
- Coumarins and an indole alkaloid from Pamburus missionisPhytochemistry, 1994
- Prenylated compounds from Atalantia racemosa: isolation and synthesis of two pyranoflavonesPhytochemistry, 1988
- 7-Phenylacetoxy coumarin from limonia crenulataPhytochemistry, 1980
- Coumarins in the RutaceaePhytochemistry, 1978