Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis

Abstract

Teles RP, Gursky LC, Faveri M, Rosa EA, Teles FRF, Feres M, Socransky SS, Haffajee AD. Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis. J Clin Periodontol 2010; 37: 313–323. doi: 10.1111/j.1600‐051X.2010.01534.x. Abstract Aim: To examine relationships between subgingival biofilm composition and levels of gingival crevicular fluid (GCF) cytokines in periodontal health and generalized aggressive periodontitis (GAP). Materials and Methods: Periodontal parameters were measured in 25 periodontally healthy and 31 GAP subjects. Subgingival plaque and GCF samples were obtained from 14 sites from each subject. Forty subgingival taxa were quantified using checkerboard DNA–DNA hybridization and the concentrations of eight GCF cytokines were measured using Luminex. Cluster analysis was used to define sites with similar subgingival microbiotas in each clinical group. Significance of differences in clinical, microbiological and immunological parameters among clusters was determined using the Kruskal–Wallis test. Results: GAP subjects had statistically significantly higher GCF levels of interleukin‐1β (IL‐1β) (ppβ/IL‐10 ratio (pβ/IL‐10 (pβ/IL‐10 ratio than periodontally healthy subjects, suggesting an imbalance between pro‐ and anti‐inflammatory cytokines in aggressive periodontitis.