Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine ofMacaca mulatta: 8-oxoguanosine in urine is a useful marker for aging
- 18 May 2012
- journal article
- Published by Taylor & Francis Ltd in Free Radical Research
- Vol. 46 (9), 1093-1098
- https://doi.org/10.3109/10715762.2012.689428
Abstract
The levels of the oxidised forms of guanosine in leukocytes, plasma and urine of Macaca mulatta were determined using a sensitive method based on high-performance liquid chromatography–triple quadruple mass spectrometry (LC–MS/MS). The amounts of 8-oxo-7,8-dihydrodeoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxoGsn), derived from DNA and RNA, respectively, increased with age in leukocytes. The measurement of the free forms of oxidised guanosine revealed similar age-dependent increases of 8-oxo-dGsn and 8-oxoGsn in both plasma and urine, which showed considerably larger amounts of 8-oxoGsn than 8-oxo-dGsn. The 8-oxoGsn content of urine could be a useful biomarker for evaluating aging, as age-dependent increases of 8-oxoGsn are more evident in urine compared to plasma and because urine samples are readily available.Keywords
This publication has 27 references indexed in Scilit:
- Extending Healthy Life Span—From Yeast to HumansScience, 2010
- Aging and defense against generation of 8-oxo-7,8-dihydro-2′-deoxyguanosine in DNAFree Radical Biology & Medicine, 2009
- Nucleic acid oxidation in Alzheimer diseaseFree Radical Biology & Medicine, 2008
- Oxidative DNA damage in mild cognitive impairment and late-stage Alzheimer's diseaseNucleic Acids Research, 2007
- Base-excision repair of oxidative DNA damageNature, 2007
- The nucleotide pool is a significant target for oxidative stressFree Radical Biology & Medicine, 2006
- Molecular devices for high fidelity of DNA replication and gene expressionProceedings of the Japan Academy, Series B, 2006
- Extracellular 8-oxo-dG as a sensitive parameter for oxidative stressin vivoandin vitroFree Radical Research, 2005
- Mechanisms of aging: an appraisal of the oxidative stress hypothesis1,2Free Radical Biology & Medicine, 2002
- Some unusual nucleic acid bases are products of hydroxyl radical oxidation of DNA and RNAMolecular Biology Reports, 1999