Epidermal growth factor enhances oocyte maturation in pigs
- 1 September 1994
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 39 (1), 30-40
- https://doi.org/10.1002/mrd.1080390106
Abstract
Epidermal Growth Factor (EGF) has been reported to stimulate nuclear maturation in porcine oocytes (Sommer et al., 1992). The objective of this experiment was to test the effect of EGF alone or in combination with gonadotropins and follicular shell coculture, on cytoplasmic maturation of oocytes in vitro. A preliminary experiment tested the effective dose of EGF for stimulation of oocyte nuclear maturation in our culture system. Nuclear maturation rates (MII%) of 67.2 ± 4.6, 81.1 ± 8.6, and 80.7 ± 5.1 for oocyte complexes cultured in the presence of 0.1, 1, and 10 ng/ml EGF, respectively, were significantly higher (P < 0.05) than for oocytes cultured in the absence of EGF (18.1 ± 9.4%). In the main experiment a 2 × 2 × 2 factorial random complete block design was used to examine the effect of EGF (1.0 ng/ml) alone or in combination with gonadotropins and follicular shell coculture on cytoplasmic maturation. Cytoplasmic maturation was evaluated by the ability of oocytes to decondense sperm nuclei after sperm penetration. EGF alone did not stimulate cytoplasmic maturation in oocytes in vitro (P > 0.05). However, EGF showed a positive interaction (P < 0.05) with gonadotropin treatment on male pronuclear formation. We conclude that EGF alone can stimulate nuclear maturation in pig oocytes, and EGF can interact with gonadotropins to enhance cytoplasmic maturation. A high level of cytoplasmic maturation of in vitro matured pig oocytes could be achieved using a combination of EGF, gonadotropin, and follicular shell supplementation to the culture environment.Keywords
This publication has 47 references indexed in Scilit:
- Effect of follicle cells and steroidogenesis on maturation and fertilization in vitro of pig oocytesJournal of Experimental Zoology, 1993
- Follicular Heterogeneity and Oocyte Maturation in Vitro in Pigs1Biology of Reproduction, 1992
- Effects of protein synthesis on maturation, sperm penetration, and pronuclear development in porcine oocytesMolecular Reproduction and Development, 1992
- Resumption of meiosis in pig oocytes cultured with cumulus and parietal granulosa cells: The effect of protein synthesis inhibitionJournal of Experimental Zoology, 1991
- Transforming growth factor‐alpha augments meiotic maturation of cumulus cell‐enclosed mouse oocytesMolecular Reproduction and Development, 1991
- Effect of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronucleus formationGamete Research, 1988
- Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and p‐aminobenzamidine blockGamete Research, 1988
- Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicleGamete Research, 1984
- Rat ovarian epidermal growth factor receptors: Characterization and hormonal regulationMolecular and Cellular Endocrinology, 1983
- CYTOPLASMIC AND NUCLEAR MATURATION OF RABBIT OOCYTES IN VITROAnnales de Biologie Animale Biochimie Biophysique, 1973