Fractionation of biological macromolecules using carrier phase ultrafiltration
- 9 May 2001
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 74 (1), 1-11
- https://doi.org/10.1002/bit.1089
Abstract
This article discusses a novel mode of operation for ultrafiltration processes termed Carrier Phase Ultrafiltration (CPUF). CPUF is based on a modification of dead-end ultrafiltration. Macromolecular fractionation using ultrafiltration is strongly influenced by operating and physicochemical parameters and requires precise “fine-tuning.” CPUF facilitates the fractionation of high value biological macromolecules at optimised conditions and has several advantages over conventional modes of ultrafiltration. In this article the fractionation of two model proteins, lysozyme (MW 14100) and myoglobin (MW 17000), by CPUF using 25-kDa MWCO polysulfone membrane is discussed. Fractionation was carried out using two different CPUF modes, 1) pulse input CPUF and 2) step input CPUF. A high recovery of pure product was obtained in each case. These results are compared with those obtained from “conventional” ultrafiltration experiments. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 1–11, 2001.Keywords
This publication has 16 references indexed in Scilit:
- Simulation study of the fractionation of proteins using ultrafiltrationJournal of Membrane Science, 2000
- Analysis of protein transport and polarization through membranes using pulsed sample injection techniqueJournal of Membrane Science, 2000
- Purification of lysozyme using ultrafiltrationBiotechnology & Bioengineering, 2000
- Protein purification by ultrafiltration with pre-treated membraneJournal of Membrane Science, 2000
- Fractionation of BSA and lysozyme using ultrafiltration: effect of pH and membrane pretreatmentJournal of Membrane Science, 1998
- Fractionation of BSA and lysozyme using ultrafiltration: Effect of gas spargingAIChE Journal, 1998
- Intermolecular Forces between Proteins and Polymer Films with Relevance to FiltrationLangmuir, 1997
- Fractionation of natural and model egg-white protein solutions with modified and unmodified polysulfone UF membranesJournal of Membrane Science, 1997
- Protein fractionation in a vortex flow filter. II: Separation of simulated mixturesJournal of Membrane Science, 1996
- Separation of proteins by surface modified polysulfone membranesJournal of Membrane Science, 1991