Kinetics of Experimental Infection of Sheep with Ehrlichia ruminantium Cultivated in Tick and Mammalian Cell Lines
- 1 January 2002
- journal article
- Published by Springer Science and Business Media LLC in Experimental and Applied Acarology
- Vol. 28 (1-4), 187-193
- https://doi.org/10.1023/a:1025390215007
Abstract
Inoculation of sheep with Ehrlichia (previously Cowdria) ruminantium which has been cultivated in mammalian endothelial cell cultures is almost always followed by a severe clinical reaction, whereas inoculation of the agent cultivated in tick cell lines usually does not provoke a clinical response, but may result in seroconversion and/or protection against subsequent challenge with virulent stabilates. A quantitative, real-time PCR assay was developed to determine the kinetics of infection (rickettsaemia) in sheep inoculated with tick cell- and mammalian cell-derived E. ruminantium (Gardel isolate). The method and initial results are described, and the significance of the findings is discussed in relation to the clinical responses of the sheep.Keywords
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