VIGR – a novel inducible adhesion family G‐protein coupled receptor in endothelial cells
- 5 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 569 (1-3), 149-155
- https://doi.org/10.1016/j.febslet.2004.05.038
Abstract
Using a signal sequence trap for selection of differentially expressed secretory and membrane proteins, we identified a novel member of the adhesion family of G-protein coupled receptors (GPCRs), termed vascular inducible GPCR (VIGR). VIGR contains C1r-C1s, Uegf and Bmp1 (CUB) and pentraxin (PTX)-like modules and a mucin-like spacer, followed by seven transmembrane domains. By surface biotinylation as well as by immunofluorescence analysis we demonstrate that endogenous, highly glycosylated VIGR is expressed on the cell surface of endothelial cells (ECs) upon LPS or thrombin treatment, and inducible expression is mediated by MAP kinases, but not NF-kappaB. We show that VIGR is selectively expressed in ECs derived from larger vessels, but not from microvessels. In summary, VIGR represents a novel GPCR of the adhesion family, which is unique in its long extra-cellular domain comprising CUB and PTX-like modules and in its inducibility by LPS and thrombin in a subset of ECs, suggesting an important function in cell-adhesion and potentially links inflammation and coagulation.Keywords
This publication has 44 references indexed in Scilit:
- The ‘magic tail’ of G protein‐coupled receptors: an anchorage for functional protein networksFEBS Letters, 2003
- Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune responseNature, 2002
- Seven-transmembrane receptorsNature Reviews Molecular Cell Biology, 2002
- EMR4, a Novel Epidermal Growth Factor (EGF)-TM7 Molecule Up-regulated in Activated Mouse Macrophages, Binds to a Putative Cellular Ligand on B Lymphoma Cell Line A20Published by Elsevier BV ,2002
- Cleavage of Ig-Hepta at a “SEA” Module and at a Conserved G Protein-coupled Receptor Proteolytic SitePublished by Elsevier BV ,2002
- LPS and Cytokine-Activated EndotheliumSeminars in Thrombosis and Hemostasis, 2000
- A Family of Heptahelical Receptors With Adhesion-Like Domains: A Marriage Between Two Super FamiliesJournal of Receptors and Signal Transduction, 2000
- Mutational Analysis of Extracellular Cysteine Residues of Rat Secretin Receptor Shows that Disulfide Bridges are Essential for Receptor FunctionJBIC Journal of Biological Inorganic Chemistry, 1997
- The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A proteinImmunology Today, 1994
- The CUB Domain: A Widespread Module in Developmentally Regulated ProteinsJournal of Molecular Biology, 1993