Isolation and characterisation of T lymphocytes from sural nerve biopsies in patients with Guillain-Barre syndrome and chronic inflammatory demyelinating polyneuropathy.
Open Access
- 1 October 1996
- journal article
- research article
- Published by BMJ in Journal of Neurology, Neurosurgery & Psychiatry
- Vol. 61 (4), 362-368
- https://doi.org/10.1136/jnnp.61.4.362
Abstract
OBJECTIVES: To characterise cultured T lymphocytes from nerve biopsies in patients with Guillain-Barré syndrome and chronic inflammatory demyelinating polyneuropathy (CIDP). METHODS: Sural nerve biopsies, obtained from six patients with Guillain-Barré syndrome, four with CIDP, and six controls with other neuropathies, were cultured with 20 U/ml recombinant interleukin-2 (IL-2) for eight weeks. Flow cytometry was used to determine the phenotype of cultured T lymphocytes. Their proliferative responses to a range of bacterial antigens were also examined. RESULTS: T cell lines were established from four of six patients with Guillain-Barré syndrome, one of four with CIDP, one patient with peripheral nerve vasculitis, and none of five controls with non-inflammatory neuropathies. One of these T cell lines from a patient with Guillain-Barré syndrome, preceded by Campylobacter jejuni infection, consisted entirely of gamma delta TCR+ T lymphocytes. The peripheral blood of this patient also contained an increased frequency of gamma delta T cells when stimulated with C jejuni. The nerve derived T cell lines failed to show a proliferative response to bacterial antigens or to a preparation of myelin proteins. CONCLUSIONS: A new technique to isolate T cells from nerve biopsies in patients with Guillain-Barré syndrome and CIDP is reported. This technique may prove to be a useful tool in the investigation of the pathogenesis of other inflammatory neuropathies such as peripheral nerve vasculitis. The isolation of a gamma delta TCR+ nerve T cell line is of interest because of the possibility that these cells might respond to glycolipid epitopes common to C jejuni and peripheral nerve gangliosides.This publication has 24 references indexed in Scilit:
- Natural and synthetic non-peptide antigens recognized by human γδ T cellsNature, 1995
- Gamma/Delta CellsAnnual Review of Immunology, 1993
- Increase of Interleukin 2 Receptor and CD45RO Antigen on Lymphocytes Cultured with Human CytomegalovirusCellular Immunology, 1993
- Lymphocyte interactions with endothelial cellsImmunology Today, 1992
- Isolation of YERSINIA‐Specific T Cell Clones from the Synovial Membrane and Synovial Fluid of a Patient with Reactive ArthritisArthritis & Rheumatism, 1991
- Clonal analysis of liver-infiltrating T cells in patients with LKM-1 antibody-positive autoimmune chronic active hepatitisClinical and Experimental Immunology, 1991
- Recognition of cluster of differentiation 1 antigens by human CD4−CD8>− cytolytic T lymphocyteNature, 1989
- Cytotoxic T lymphocytes specific for self tumor immunoglobulin express T cell receptor delta chain.The Journal of Experimental Medicine, 1989
- Two forms of the T-cell receptor γ protein found on peripheral blood cytotoxic T lymphocytesNature, 1987
- Genetically restricted suppressor T-cell clones derived from lepromatous leprosy lesionsNature, 1986