Biomimetic Silica Microspheres in Biosensing
Open Access
- 17 March 2010
- Vol. 15 (3), 1932-1957
- https://doi.org/10.3390/molecules15031932
Abstract
Lipid vesicles spontaneously fuse and assemble into a lipid bilayer on planar or spherical silica surfaces and other substrates. The supported lipid bilayers (SLBs) maintain characteristics of biological membranes, and are thus considered to be biomembrane mimetic systems that are stable because of the underlying substrate. Examples of their shared characteristics with biomembranes include lateral fluidity, barrier formation to ions and molecules, and their ability to incorporate membrane proteins into them. Biomimetic silica microspheres consisting of SLBs on solid or porous silica microspheres have been utilized for different biosensing applications. The advantages of such biomimetic microspheres for biosensing include their increased surface area to volume ratio which improves the detection limits of analytes, and their amenability for miniaturization, multiplexing and high throughput screening. This review presents examples and formats of using such biomimetic solid or porous silica microspheres in biosensing.This publication has 98 references indexed in Scilit:
- Supported Bilayers Formed from Different Phospholipids on Spherical Silica SubstratesLangmuir, 2009
- An overview of lipid membrane supported by colloidal particlesAdvances in Colloid and Interface Science, 2007
- Characterization of Physical Properties of Supported Phospholipid Membranes Using Imaging Ellipsometry at Optical WavelengthsBiophysical Journal, 2007
- Superquenching as a detector for microsphere-based flow cytometric assaysCytometry Part A, 2006
- Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared InvestigationsLangmuir, 2004
- Applications of Fluorescent Polymer Superquenching to High Throughput Screening Assays for Protein KinasesASSAY and Drug Development Technologies, 2004
- Submicrometric Lipobead-Based Fluorescence Sensors for Chloride Ion Measurements in Aqueous SolutionAnalytical Chemistry, 2003
- Detection of Single Nucleotide Mismatches via Fluorescent Polymer SuperquenchingLangmuir, 2003
- Monodisperse Mesoporous Silica Microspheres Formed by Evaporation-Induced Self Assembly of Surfactant Templates in AerosolsAdvanced Materials, 2002
- Detection of DNA Hybridization via Fluorescent Polymer SuperquenchingLangmuir, 2002